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tissue lymphocyte isolation kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science tissue lymphocyte isolation kit
    Tissue Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+tumor+infiltrating+lymphocyte+isolation+kit/pmc12967015-214-3-10?v=Beijing+Solarbio+Science
    Average 95 stars, based on 47 article reviews
    tissue lymphocyte isolation kit - by Bioz Stars, 2026-07
    95/100 stars

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    MEKi+RT improved the function of infiltrated T cells. (A) Diagram of treatment procedures after LLC cell inoculation in mice. (B) Tumor growth curves for four groups. (C, D) Flow cytometry analysis of the CD8+ and CD4+ T lymphocyte proportions in infiltrating immune cells. (E–I) Flow cytometry analysis of IFN-γ+ CD4+ T lymphocytes and CD107a+, granzyme B, perforin, and IFN-γ+ CD8+ T lymphocytes. (J) Immunofluorescence of CD8, CD4 T lymphocytes, and CXCL10 in tumor-infiltrating cells across groups. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. IFN, interferon; MEK inhibitor; RT, radiotherapy.

    Journal: Frontiers in Immunology

    Article Title: Impact of MEK inhibition on T-cell infiltration and function after radiotherapy in KRAS-mutant lung cancer

    doi: 10.3389/fimmu.2025.1663502

    Figure Lengend Snippet: MEKi+RT improved the function of infiltrated T cells. (A) Diagram of treatment procedures after LLC cell inoculation in mice. (B) Tumor growth curves for four groups. (C, D) Flow cytometry analysis of the CD8+ and CD4+ T lymphocyte proportions in infiltrating immune cells. (E–I) Flow cytometry analysis of IFN-γ+ CD4+ T lymphocytes and CD107a+, granzyme B, perforin, and IFN-γ+ CD8+ T lymphocytes. (J) Immunofluorescence of CD8, CD4 T lymphocytes, and CXCL10 in tumor-infiltrating cells across groups. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. IFN, interferon; MEK inhibitor; RT, radiotherapy.

    Article Snippet: The resultant cell suspension was either collected directly or isolated using the Mouse Tumor Infiltrating Lymphocyte Isolation Kit (Solarbio, Beijing, China; Cat. No. P9000) in accordance with the manufacturer’s protocol.

    Techniques: Flow Cytometry, Immunofluorescence

    The treatment model with low-dose radiation in the mouse model. (A) Diagram of treatment procedures after LLC cell inoculation in mice. (B) Mice injected with LLC cells were categorized into control, MEKi, RT, and MEKi+RT groups, with tumor sizes measured on the left and right side. (C) Tumor growth curves for four groups. (D) Tumor weight comparison post-excision. (E, F) Flow cytometry analysis of CD8+ and CD4+ T lymphocyte proportions in infiltrating immune cells of the tumor on the left side. (G, H) Flow cytometry analysis of CD8+ and CD4+ T lymphocyte proportions in spleen cells. (I–L) Flow cytometry analysis of CD107a+, granzyme B, and IFN-γ+ CD8+ T lymphocytes and IFN-γ+ CD4+ T lymphocytes. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. IFN, interferon; MEK inhibitor; RT, radiotherapy.

    Journal: Frontiers in Immunology

    Article Title: Impact of MEK inhibition on T-cell infiltration and function after radiotherapy in KRAS-mutant lung cancer

    doi: 10.3389/fimmu.2025.1663502

    Figure Lengend Snippet: The treatment model with low-dose radiation in the mouse model. (A) Diagram of treatment procedures after LLC cell inoculation in mice. (B) Mice injected with LLC cells were categorized into control, MEKi, RT, and MEKi+RT groups, with tumor sizes measured on the left and right side. (C) Tumor growth curves for four groups. (D) Tumor weight comparison post-excision. (E, F) Flow cytometry analysis of CD8+ and CD4+ T lymphocyte proportions in infiltrating immune cells of the tumor on the left side. (G, H) Flow cytometry analysis of CD8+ and CD4+ T lymphocyte proportions in spleen cells. (I–L) Flow cytometry analysis of CD107a+, granzyme B, and IFN-γ+ CD8+ T lymphocytes and IFN-γ+ CD4+ T lymphocytes. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. IFN, interferon; MEK inhibitor; RT, radiotherapy.

    Article Snippet: The resultant cell suspension was either collected directly or isolated using the Mouse Tumor Infiltrating Lymphocyte Isolation Kit (Solarbio, Beijing, China; Cat. No. P9000) in accordance with the manufacturer’s protocol.

    Techniques: Injection, Control, Comparison, Flow Cytometry